Xiao-Wen Chen and Nien-Tsu Huang, “Dual Ion-Selective Membrane Deposited Ion-Sensitive Field-Effect Transistor Integrating a Whole Blood Processing Microchamber for In Situ Blood Ion Testing,” ACS Sensors, 8 (2), 904-913, 2023
Zhi-Xuan Lai, Chia-Chien Wu and Nien-Tsu Huang, “A Microfluidic Platform with an Embedded Miniaturized Electrochemical Sensor for On-Chip Plasma Extraction Followed by In Situ High-Sensitivity C-Reactive Protein (hs-CRP) Detection,” Biosensors, 12 (12), 1163, 2022
Chia-Yu Hsieh and Nien-Tsu Huang, “A proton-selective membrane (PSM)-deposited dual-gate ion-sensitive field-effect transistor (DG-ISFET) integrating a microchamber-embedded filter membrane for bacterial enrichment and antimicrobial susceptibility test,” Sensors and Actuators B: chemical, 359, 131580, 2022
Cheng-Chieh Liao, Yi-Zih Chen, Shang-Jyun Lin, Ho-Wen Cheng, Juen-Kai Wang, Yuh-Lin Wang, Yi-Yin Han, Nien-Tsu Huang, “A microfluidic microwell device operated by the automated microfluidic control system for surface-enhanced Raman scattering-based antimicrobial susceptibility testing,” Biosensors and Bioelectronics, 2021, 113483, 2021
Yi-Hsin Tai, Shu-Cheng Lo, Kevin Montagne, Po-Cheng Tsai, Cheng-Chieh Liao, Sheng-Hann Wang, Iuan-Sheau Chin, Di Xing, Ya-Lun Ho, Nien-Tsu Huang, Pei-Kuen Wei, Jean-Jacques Delaunay, “Enhancing Raman signals from bacteria using dielectrophoretic force between conductive lensed fiber and black silicon,” Biosensors and Bioelectronics, 19, 113463, 2021
Sheng-Han Chu, Li-Lun Lo, Richard Lee Lai, T. Tony Yang, Rueyhung Roc Weng, Jung-Chi Liao, and Nien-Tsu Huang, “A microfluidic device for in situ fixation and super-resolved mechanosensation studies of primary cilia,” Biomicrofluidics, 13, 014105, 2019
Kai-Wei Chang, Ho-Wen Cheng, Jessie Shiue, Juen-Kai Wang, Yuh-Lin Wang, Nien-Tsu Huang, “Antibiotic Susceptibility Test with Surface-Enhanced Raman Scattering in a Microfluidic System,” Analytical Chemistry, 91, 10988-10995, 2019
Richard Lee Lai, and Nien-Tsu Huang, “Dimensional analysis and parametric studies of the microwell for particle trapping,” Microfluidics and Nanofluidics, 23:121, 2019
Da-Han Kuan, Chia-Chien Wu, Wei-Yu Su, and Nien-Tsu Huang, “A Microfluidic Device for simultaneous Extraction of Plasma, Red Blood Cells and on-chip White Blood Cell Trapping,” Scientific Reports, 8, 15345, Oct. 2018
S.-H. Huang, Y.-S. Chang, J.-M. J. Juang, K.-W. Chang, M.-H. Tsai, T.-P. Lu, L.-C. Lai, E. Y. Chuang, and N.-T. Huang, “An Automated Microfluidic DNA Microarray Platform for Genetic Variants Detection in Inherited Arrhythmic Diseases,” Analyst, 143, 1367-1377, Mar. 2018
N.-T. Huang*, Y. J. Hwong, R. L. Lai, “A microfluidic microwell device for immunomagnetic single-cell trapping,” Microfluidics and Nanofluidics, 22, 16, 2018
J.-K. Lee, I. –S. Wang, C.-H. Huang, Y.-F. Chen, N.-T. Huang, C.-T. Lin, “Pre-Clinical Tests of an Integrated CMOS Biomolecular Sensor for Cardiac Diseases Diagnosis,” Sensors, 17(12), 2733, Nov. 2017
Y. C. Wu, N. Lee, Y.K. Tu, C.P. Huang, N. T. Huang, Y.F. Chen, P.C. Chang, “ “Salivary biomarker combination prediction model for the diagnosis of periodontitis in a Taiwanese population,” J. Formos. Med. Assoc., 117(9), 841-848, Nov. 2017
H. T.-H. Lin, C.-K. Yang, C.-C. Lin, A. M.-H. Wu, L. A. Wang, and N.-T. Huang, “A Large-Area Nanoplasmonic Sensor Fabricated by Rapid Thermal Annealing Treatment for Label-Free and Multi-Point Immunoglobulin Sensing,” Nanomaterials, 7(5), 100, May 2017
D.-H. Kuan, I.-S. Wang, J.-R. Lin, C.-H. Yang, C.-H. Huang, Y.-H. Lin, C.-T. Lin and N.-T. Huang, “A microfluidic device integrating dual CMOS polysilicon nanowire sensors for on-chip whole blood processing and the simultaneous detection of multiple analytes,” Lab on a Chip, 16, 3105-3113, Aug. 2016
P. Chen, N.-T. Huang, M.-T. Chung, T. T. Cornell, and K Kurabayashi, “Label-free cytokine micro- and nano-biosensing towards personalized medicine of systemic inflammatory disorders,” Advanced Drug Delivery Reviews, 95, 90-103, Dec. 2015
1. A. B. Simon, J. P. Frampton, N. -T. Huang, S. Paczesny, K. Kurabayashi, S. Takayama,, “Aqueous two-phase systems enable multiplexing of homogeneous immunoassays,” TECHNOLOGY, 2, 176-184, Jun. 2014
N.–T. Huang, H. Zhang, M. –T. Chung, J. H. Seo, and K. Kurabayashi, “Recent Advancements in Optofluidics-Based Single-cell Analysis: Fully Optical On-Chip Cellular Manipulation, Treatment, and Property Detection,” Lab on a Chip, 14, 1230-1245, Mar. 2014
B.-R. Oh, N.-T. Huang, W. Chen, J. Seo, P. Chen, T. T. Cornell, T. P. Shanley, J. Fu, and K. Kurabayashi, “Integrated Nanoplasmonic Sensing for Cellular Functional Immunoanalysis Using Human Blood,” ACS Nano, 8, 2667-2676, Feb. 2014
W. Chen*, N. -T. Huang*, B. -R. Oh, R. H. W. Lam, R. Fan, T. T. Cornell, T. P. Shanley, K. Kurabayashi, and J. Fu, “Surface-micromachined microfiltration membranes for efficient isolation and functional immunophenotyping of subpopulations of immune cells,” Advanced Healthcare Materials, 2(7), 965-975, Jul. 2013
W. Chen*, N.-T. Huang*, X. Li, Z. Yu, K. Kurabayashi, and J. Fu, “Emerging Microfluidic Tools for Functional Immunophenotyping: A New Potential Paradigm for Immune Status Characterization,” Frontiers in Oncology, 3, 98, Apr. 2013
N. -T. Huang*, W. Chen*, B. -R. Oh, T. T. Cornell, T. P. Shanley, J. Fu, and K. Kurabayashi, “An integrated microfluidic platform for in-situ cellular cytokine secretion immunophenotyping,” Lab on a Chip, 12, 4093-4101, Jul. 2012
Y. -C. Tung, N. -T. Huang, B. -R. Oh, B. Patra, C. -C. Pan, T. Qiu, P. K. Chu, W. Zhang, and K. Kurabayashi, “Optofluidic detection for cellular phenotyping,” Lab on a Chip, 12, 3552-3565, Jun. 2012
N. -T. Huang, S. C. Truxal, Y. -C. Tung, A. Y. Hsiao, G. D. Luker, S. Takayama, and K. Kurabayashi, “Multiplexed spectral signature detection for microfluidic color-coded bioparticle flow,” Analytical Chemistry, 82, 9506-9512, Oct. 2010
N. -T. Huang, S. C. Truxal, Y. -C. Tung, A. Hsiao, S. Takayama, and K. Kurabayashi, “High-Speed Tuning of Visible Laser Wavelength Using a Nanoimprinted Grating Optical Tunable Filter,” Applied Physics Letters, 95, 21106, Nov. 2009
Chi-Yao Ku, Nien-Tsu Huang, “Integration of air-Liquid microfluidics Integrating Sers Substrate for Bacteria Identification Based on Adsorptive Separation,” IEEE NEMS 2024, Kyoto, Japan, May 2024
Ming-Chun Lin, Nien-Tsu Huang, “Integration of Free Flow Electrophoresis and Surface-Enhanced Raman Scattering for Multiplex Biomolecule Analysis,” IEEE NEMS 2024, Kyoto, Japan, May 2024
Yen-An Chen, Nien-Tsu Huang, “A Microfluidic Platform Integrating Electrochemical Sensors for on-Chip Whole Blood Processing and in-situ Dual Cardiac Vascular Disease Biomarker Detection,” IEEE NEMS 2024, Kyoto, Japan, May 2024
Syuan-Rong Huang, Nien-Tsu Huang, “Aptamer-functionalized Extended Gate Field-Effect Transistor (EGFET) Integrating Whole-Blood Processing Microfluidics for Troponin I Detection,” IEEE NEMS 2024, Kyoto, Japan, May 2024
Tzu-Yu Liu, Nien-Tsu Huang, “Ion Selective Membrane with Dual-Gate Ion-Sensitive Field-Effect Transistor Integrating the Microfluidic Channel for Heavy Metal Ions Test in Wastewater,” IEEE NEMS 2024, Kyoto, Japan, May 2024
Kuan-Chun Yeh, Hsiang-Yu Wang, Nien-Tsu Huang, “The Anodic Aluminum Oxide Templates and Etching-Based Rapid Thermal Annealing for Fabricating Localized Surface Plasmon Resonance Sensors Integrating Micro-Channel for C-Reactive Protein Detection,” IEEE NEMS 2024, Kyoto, Japan, May 2024
Chia-Pei Wang, Kuang-Hsing Chiang, Nien-Tsu Huang, “A Microfluidic Platform for Collective Endothelial Cell Migration Assay Under Glucose Gradient and Cyclic Hypoxia Stimuli,” IEEE NEMS 2024, Kyoto, Japan, May 2024
Min-Yen Hsin, and Yen-Chen Hsiung, Kuang-Hsing Chiang, and Nien-Tsu Huang, “A Microfluidic Device to Simultaneously Generate Shear Stress and Hypoxia Conditions to Study Endotheilial Cell Morphology and Reactive Oxygen Species Behavior,” µTAS 2023, Katowice, Poland, Oct. 2023
Po-Hsuan Chao and Nien-Tsu Huang, “The Microfluidic Microwell array integrAting Surface Enhanced raman scattering (SERS) Platform assisted with Machine Learning for Bacteria strain identification,” Transducers 2023, Kyoto, Japan, Jun. 2023
Chia-Chin Hsieh and Nien-Tsu Huang, “The anodic aluminum oxide (AAO) template fabricated Localized Surface Plasmon Resonance (LSPR) sensor for the plasmonic coupling effect study,” IEEE NEMS 2023, Jeju, Korea, May 2023
Xiao-Wen Chen, Syuan-Rong Huang, and Nien-Tsu Huang, “Dual Ion-Selective Membrane Deposited Ion-Sensitive Field-Effect Transistor (DISM-ISFET) integrating Whole Blood Processing Microchamber for in situ Blood Ion Testing,” 2023 IEEE 36rd International Conference on Micro Electro Mechanical Systems (MEMS), Munich, Germany, Jan. 2023
Shang-Jyun Lin, Po-Hsuan Chao, Nien-Tsu Huang, “A microfluidic antibiotic concentration gradient generator integrating surface-enhanced Raman spectroscopy for multiparallel antimicrobial susceptibility testing,” µTAS 2021, Palm Springs, USA, Oct. 2021
Zhi-Xuan Lai, and Nien-Tsu Huang, “A microfluidic platform for undiluted plasma separation and electrochemical detection of C-reactive protein,” µTAS 2021, Palm Springs, USA, Oct. 2021
Zhi-Xuan Lai and Nien-Tsu Huang, “An Electrochemical Impedance Spectroscopy Biosensor Embedded Microfluidics for Whole Blood Processing and Biomarkers Detection,” ICSS conference, Kaohsiung, Taiwan, Oct. 2020
Shang-Jyun Lin, Cheng-Chieh Liao, Yi-Zih Chen and Nien-Tsu Huang, “An Automatic Medium Exchanging Microwell Microfluidic Device for SERS-based Antibiotics Susceptibility Test,” ICSS conference, Kaohsiung, Taiwan, Oct. 2020
Pin-Fan. Chen, and Nien-Tsu Huang, “Nanoplasmonic Sensor Fabricated by Electron Beam Lithography Integrated with Automated Microfluidic System for Biomolecule Detection,” ICSS conference, Kaohsiung, Taiwan, Oct. 2020
Y.H. Huang, C.Y. Hsieh and Nien-Tsu Huang, “A Reference Electrode Embedded in Dual-gated Ion-Sensitive Field-Effect Transistor for Antibiotic Susceptibility Test,” ICSS conference, Kaohsiung, Taiwan, Oct. 2020
C. Liao, H. Huang, Y. Chen and N. Huang, “The Microfluidic Microwell Device Integrating Surface Enhanced Raman Scattering for Bacteria Enrichment and in Situ Antibiotic Susceptibility Test,” 2020 IEEE 33rd International Conference on Micro Electro Mechanical Systems (MEMS), 1048-1051, Vancouver, BC, Canada, Jan. 2020
Sheng-Han Chu, and Nien-Tsu Huang, “Determining mechanical stimulation responses of primary cilia with an integrated microfluidic platform,” µTAS 2019, Basel, Switzerland, Oct. 2019
Kai-Wei Chang, and Nien-Tsu Huang, “A Microfluidic System Integrating Membrane Filtration and Surface-Enhanced Raman Scattering for Rapid Antibiotic Susceptibility test,” µTAS 2019, Basel, Switzerland, Oct. 2019
C.-C. Lin, J.-S. Chen, C.-L. Wu, L. A. Wang, N.-T. Huang, “A Nanodisk Array Based Localized Surface Plasmon Resonance (LSPR) Sensor Fabricated by Laser Interference Lithography,” IEEE NEMS 2019, Bangkok, Thailand, Apr. 2019
Hsiu-Kang Huang, Nien-Tsu Huang, “A Microfluidic Microwell Device Integrating Surface-enhanced Raman Scattering for Rapid Antibiotic Susceptibility Test of Blood-Borne Pathogen,” IEEE NEMS 2019, Bangkok, Thailand, Apr. 2019
Kai-Wei Chang, Ho-Wen Cheng, Jessie Shiue, Juen-Kai Wang, Yuh-Lin Wang, and Nien-Tsu Huang, “Rapid bacterial detection using a microfluidic system integrating membrane filtration and surface-enhanced Raman scattering,” µTAS 2018, Kaohsiung, Taiwan, Nov. 2018
Hana Tzu-Han Lin, Jhih-Siang Chen, and Nien-Tsu Huang, “An automated microfluidic platform integrating dual-mode microchannel and localized surface plasmon resonance sensing for multi-parallel biomolecule detection,” µTAS 2018, Kaohsiung, Taiwan, Nov. 2018
Sheng-Han Chu, Li-Lun Lo, Richard Lee Lai, T. Tony Yang, Jung-Chi Liao and Nien-Tsu Huang, “Determining mechanical stimulation responses of primary cilia with an integrated microfluidic platform,” µTAS 2018, Kaohsiung, Taiwan, Nov. 2018
Chia-Chien Wu, Da-Han Kuan, Wei-Yu Su, and Nien-Tsu Huang, “A Microfluidic Device for Simultaneous Extraction of Plasma, Red Blood Cells and On-Chip White Blood Cells Trapping,” µTAS 2018, Kaohsiung, Taiwan, Nov. 2018
Da-Han Kuan, Chia-Chien Wu, Ting-Wei Lin, Chih-Ting Lin, and Nien-Tsu Huang, “A microfluidic platform for whole blood collection and on-chip plasma extraction,” µTAS 2018, Kaohsiung, Taiwan, Nov. 2018
C.-C. Lin, J.-F. Luo, L. A. Wang, and N.-T. Huang, “A Nanoplasmonic Sensor Fabricated by Laser Interference Lithography (LIL) for Immunoglobulin Detection,” CLEO: Applications and Technology 2018, May 2018
Yuh-Jen Hwong, Richard Lee Lai, and Nien-Tsu Huang, “A Microfluidic Device Integrating Microwell with a Permanent Magnet for Immunomagnetic Single Cell Trapping,” µTAS 2017, Savannah, Georgia, Oct. 2017
Shu-Hong Huang, Yu-Shin Chang, Kai-Wei Chang, Mong-Hsun Tsai, Nien-Tsu Huang, “An Automatic Microfluidic DNA Microarray Platform for SNP Detection Using a DNA Intercalating Dye and Graphene Oxide,” µTAS 2017, Savannah, Georgia, Oct. 2017
Yuh-Jen Hwong, Nien-Tsu Huang, “A microfluidic device with hydrodynamic trap arrays for white blood cell counting in peritoneal dialysis solution,” 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC’17), Jeju Island, Korea, Jul. 2017
H. T.-H. Lin, C.-C. Lin, and N.-T. Huang, “Localized surface plasmon resonance platform for multi-point and real-time biosensing,” CLEO: Applications and Technology 2017, May 2017
Da-Han Kuan, I-Shun Wang, Chih-Ting Lin, Nien-Tsu Huang, “A microfluidic device integrating dual CMOS polysilicon nanowire sensors for on-chip whole blood processing and the simultaneous detection of multiple analytes,” µTAS 2016, Dublin, Ireland, Oct. 2016
W. H. Jheng, L. A. Wang and N. T. Huang, “Large quantity optical fiber probes fabrication intended for surface plasmon resonance applications,” 2015 International Conference on Optical MEMS and Nanophotonics (OMN), 1-2, Jerusalem, Israel, Aug. 2015
Yu-Shin Chang, Frank Shyu, Kai-Wei Chang, Mon-Hsun Tsai, Nien-Tsu Huang, “Point Mutation Detection by Microfluidic DNA Microarray for Long QT Syndrome,” Optofluidics 2015, Taipei, Taiwan, Jul. 2015
Yeu-Farn Shih, Nien-Tsu Huang, Chih-Kung Lee, “Capturing CD4 cells using a functionalized circular microfluidic device and glutaraldehyde as biolinker for tuberculosis detection and diagnosis,” SPIE Phonics West, San Francisco, USA, Feb. 2015
Weiqiang Chen, Nien-Tsu Huang, Bo-Ram Oh, Timothy T. Cornell, Thomas P. Shanley, Katsuo Kurabayashi, and Jianping Fu, “Microfluidic immunophenotyping assay platform for immunomonitoring of subpopulations of immune cells,” Proc. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2013), 1761-1763, Freiburg, Germany, Oct. 2013
Bo-Ram Oh, Nien-Tsu Huang, Weiqiang Chen, Jungwhan Seo, Jianping Fu, and Katsuo Kurabayashi, “Localized surface plasmon resonance (LSPR) optofluidic biosensor for label-free cellular immunophenotyping,” Proc. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2013), 92-94, Freiburg, Germany, Oct. 2013
N. -T. Huang, W. Chen, B. -R. Oh, J. Fu, and K. Kurabayashi, “An integrated microfluidic platform for in-situ cellular cytokine secretion immunophenotyping,” The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2012, Okinawa, Japan, Nov. 2012
W. Chen, N. -T. Huang, K. Kurabayashi, and J. Fu, “Surface Micromaching Polydimethylsiloxane(PDMS) for Microfluidic Biomedical Application,” The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2012, Okinawa, Japan, Nov. 2012
N. -T. Huang, S. Truxal, Y. -C. Tung, and K. Kurabayashi, “Multi-Spectral Tunable Excitation Fluorescence Microscopy with a Nanoimprinted PDMS-on-Silicon Grating Optical Filter,” The 25th International Conference on Micro Electro Mechanical Systems IEEE MEMS 2012, 648-651, Paris, France, Jan. 2012
S. C. Truxal, N. -T. Huang and K. Kurabayashi, “A Nano Grating Tunable MEMS Optical Filter for High-Speed On-Chip Multispectral Fluorescent Detection,” Proc. the 31st Annual International Conference of IEEE Engineering in Medicine and Biology Society, 6693-6695, Minneapolis, MN, Sept. 2009
W. -L. Hsu, S. -S. Lee, N. -T. Huang, Y. -W. Chen, Y. -W. Chan, C. -K. Lee, W. -R. Lai, and Y.- H. Chen, “Ellipsometer Surface Plasma Resonance,” 2005 International Conference on Mechatronics (ICM 2005), Taipei, Taiwan, Jul. 2005
J. -W. Huang, N. -T. Huang, D. -L. Yang, S. -N. Chen, Y. -W. Chen, and C. -K. Lee, “A Design of Reflective F-θ Lens,” Proceedings of 2nd Asian & Pacific Rim Symposium on BioPhotonics 2004 (APBP 2004), 171-172, Taipei, Taiwan, Dec. 2004
S. -S. Lee, W. -L. Hsu, N. -T. Huang, C. -K. Lee, “Opto-BioMorphin: Multifunctional Opto-electronic Biochip Systems,” AOI Forum & Exhibition, Hsinchu, Taiwan, Oct. 2004
Books:
K. Kurabayashi, N. -T. Huang, and Y.-C. Tung, “Optical Nano and Micro Actuator Technology, Chapter 16: Multiscale, Hierarchical Integration of Soft Polymer Micro- and Nano Structures into Optical MEMS,” CRC press, USA, 639 pages, Nov. 2012, ISBN:9781439840535
S. -S. Lee, N. -T. Huang, Y. -W. Chen, C. -H. Lee and C. -K. Lee, “Micro Electro Mechanical Systems Technology & Applications, Chapter 11-1~11-4: Scanning Technology,” National Science Council, Instrument Technology Research Center, p. 843-850 pages, 2003
Patents:
Yeaun Jau Liou, Nien-Tsu Huang, “Multi-Channel Microfluidic Device and Method for Using the Same,” U.S. patent (US20190091684A1), 2020
S. Takayama, A. Simon, N. -T. Huang and K. Kurabayashi, “Solution Microarrays and uses thereof,” U.S. patent (US8969256 B2), 2015